Journal
BRAIN BEHAVIOR AND IMMUNITY
Volume 25, Issue 6, Pages 1170-1181Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbi.2011.03.007
Keywords
Multiple sclerosis; Th17; IL-27; IFN-beta; Experimental autoimmune encephalomyelitis; Dendritic cell; CD4(+) T cell
Categories
Funding
- Bayer Schering
- Biogen Idec.
- Science Foundation Ireland
- Irish Health Research Board
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Interferon (IFN)-beta is a commonly used therapy for relapsing remitting multiple sclerosis (RRMS). However its protective mechanism is still unclear and the failure of many patients to respond has not been explained. We have found that IFN-beta suppressed IL-23 and IL-beta production and increased IL-10 production by human dendritic cells (DC) activated with the TLR2 and dectin-1 agonist zymosan. Furthermore, IFN-beta impaired the ability of DC to promote IL-17 production by CD4(+) T cells, but did not affect IFN-gamma production. IFN-beta induced IL-27 expression by DC, and neutralisation of IL-27 abrogated the suppressive effects of IFN-beta on zymosan-induced IL-1 and IL-23 production and the generation of Th17 cells in vitro. Complementary in vivo studies in a mouse model showed that treatment with IFN-beta enhanced expression of IL-27, and reduced IL-17 in the CNS and periphery and attenuated the clinical signs of experimental autoimmune encephalomyelitis (EAE). In addition, the significant suppressive effect of IFN-beta on the ability of DC to promote Th17 cells was lost in cells from IL-27 receptor deficient mice. Finally, we showed that PBMC from non-responder RAMS patients produced significantly less IL-27 in response to IFN-beta than patients who responded to IFN-beta therapy. Our findings suggest that IFN-beta mediates its therapeutic effects in MS at least in part via the induction of IL-27, and that IL-27 may represent an alternative therapy for MS patients that do not respond to IFN-beta. (C) 2011 Elsevier Inc. All rights reserved.
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