Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 12, Pages 6180-6185Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b01656
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Funding
- Collaborative Innovation Center of Suzhou Nano Science and Technology
- Major Program of Development Foundation of Hefei Center for Physical Science and Technology
- Hefei Science Center of CAS [2015SRG-HSG037]
- National Natural Science Foundation of China [21175122, 21375121]
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There has been no report on enzyme-controlled disassembly of self-quenched NIR fluorescent nanoparticles turning fluorescence on for specific detection/imaging of the enzyme's activity in vitro and in vivo. Herein, we, reported the rational design of new NIR probe 1 whose fluorescence signal was self-quenched upon reduction-controlled condensation and subsequent assembly of its nanoparticles (i.e., 1-NPs). Then disassembly of 1-NPs by furin turned the fluorescence on. Employing this enzymatic strategy, we successfully applied 1-NPs for NIR detection of furin in vitro and NIR imaging furin activity in living cells. Moreover, we also applied 1-NPs for discriminative NIR imaging of MDA-MB-468 tumors in nude mice. This NIR probe 1 might be further developed for tumor-targeted imaging in routine preclinical studies or even in patients in the future.
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