Journal
BRAIN
Volume 135, Issue -, Pages 2155-2168Publisher
OXFORD UNIV PRESS
DOI: 10.1093/brain/aws127
Keywords
Alzheimer's disease; apolipoprotein E; synapse; array tomography; oligomeric amyloid-beta
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Funding
- NIH [K99 (AG033670-01A1), P50 AG005134, AG12406, AG08487, T32 GM07753]
- Alzheimer's disease Drug Discovery Foundation/Association for Frontotemporal Dementias
- Harvard Biophysics Training Program
- Paul and Daisy Soros Foundation
- Ellison/American Federation for Aging Research Postdoctoral Fellowship [2009A059868]
- American Health Assistance Foundation Alzheimer's Disease Fellowship
- Harvard Medical Scientist Training Program
- Grants-in-Aid for Scientific Research [24800015] Funding Source: KAKEN
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The apolipoprotein E epsilon 4 gene is the most important genetic risk factor for sporadic Alzheimer's disease, but the link between this gene and neurodegeneration remains unclear. Using array tomography, we analysed > 50 000 synapses in brains of 11 patients with Alzheimer's disease and five non-demented control subjects and found that synapse loss around senile plaques in Alzheimer's disease correlates with the burden of oligomeric amyloid-beta in the neuropil and that this synaptotoxic oligomerized peptide is present at a subset of synapses. Further analysis reveals apolipoprotein E epsilon 4 patients with Alzheimer's disease have significantly higher oligomeric amyloid-beta burden and exacerbated synapse loss around plaques compared with apolipoprotein E epsilon 3 patients. Apolipoprotein E4 protein colocalizes with oligomeric amyloid-beta and enhances synaptic localization of oligomeric amyloid-beta by > 5-fold. Biochemical characterization shows that the amyloid-beta enriched at synapses by apolipoprotein E4 includes sodium dodecyl sulphate-stable dimers and trimers. In mouse primary neuronal culture, lipidated apolipoprotein E4 enhances oligomeric amyloid-beta association with synapses via a mechanism involving apolipoprotein E receptors. Together, these data suggest that apolipoprotein E4 is a co-factor that enhances the toxicity of oligomeric amyloid-beta both by increasing its levels and directing it to synapses, providing a link between apolipoprotein E epsilon 4 genotype and synapse loss, a major correlate of cognitive decline in Alzheimer's disease.
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