Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 7, Pages 4037-4041Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b00375
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Funding
- National Basic Research Program of China (973 program) [2015CB932600, 2013CB933000]
- National Natural Science Foundation of China [21375042, 21405054]
- 1000 Young Talent
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Almost all of the important functions of DNA are realized by proteins which interact with specific DNA, which actually happens in a limited space. However, most of the studies about the protein-DNA binding are in an unconfined space. Here, we propose a new method, nanopore-based DNA-probe sequence-evolution (NDPSE), which includes up to 6 different DNA-probe systems successively designed in a nanoscale confined space which unveil the more realistic characteristics of protein-DNA binding phenomena. There are several features; for example, first, the edge-hindrance and core-hindrance contribute differently for the binding events, and second, there is an equilibrium between protein-DNA binding and DNA-DNA hybridization.
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