Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 9, Pages 4925-4932Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b00569
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- Ministry of Science and Technology of Taiwan [NSC 101-2113-M-002-002-MY3]
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We have developed a simple, sensitive, and rapid fluorescence assay for the detection of cancer cells, based on turn-on retro-self-quenched fluorescence inside the cells. 1,3-Phenylenediamine resin (DAR) nano-particles (NPs) containing rhodamine 6G (R6G) are conjugated with aptamer (apt) sgc8c to prepare sgc8c-R6GDAR NPs, while that containing rhodamine 101 (R101) are conjugated with TD05 for the preparation of TD05-R101DAR NPs. The sgc8c-R6GDAR and TD05-R101DAR NPs separately recognize CCRF-CEM and Ramos cells. The fluorescence intensities of the two apt-DAR NPs are both weak due to self-quenching, but they increase inside the cells as a result of release of the fluorophores from the apt-DAR NPs. The apt-DAR NPs' structure becomes less compact at low pH value, leading to the release of the fluorophores. The sgc8c-R6GDAR and TD05-R101DAR NPs allow detection of as low as 44 CCRF-CEM cells and 79 Ramos cells mL(-1), respectively, using a commercial reader within 10 min. Practicality of the two probes have been validated by the quantitation and identification of CCRF-CEM and Ramos cells spiked in blood samples through conventional fluorescence and flow cytometry analysis, with advantages of sensitivity, selectivity, and rapidity.
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