Journal
BONE MARROW TRANSPLANTATION
Volume 47, Issue 11, Pages 1419-1427Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/bmt.2012.43
Keywords
haploidentical SCT; immune reconstitution; T-cell-depleted graft; natural killer cells; dendritic cells
Categories
Funding
- National Health Service of Spain [FIS PS09/02393]
- Fundacion Mutua Madrilena
- 'La hucha de Tomas' project
Ask authors/readers for more resources
Graft engineering procedures for hematopoietic SCT (HSCT) may improve the chance of success in matched unrelated donor (MUD) and haploidentical donor transplantations. Successful donor immune reconstitution is important to mediate GVL effects in reduced-intensity conditioning (RIC) HSCT. We prospectively investigated early immune reconstitution and clinical outcome in 30 CD3/CD19-depleted MUD (n = 15) or HP (n = 15) HSCTs for high-risk childhood leukemia using a fludarabine-based RIC without serotherapy. The graft consisted of a mean of 10.5 x 10(6)/kg CD34+, 77 x 10(3)/kg CD3+ and 39 x 10(6)/kg CD56+ cells. After transplantation, 86% of the patients engrafted. In all, 13% of patients had >grade 3 acute GVHD. Natural killer (NK) cell, DC and T-cell recovery achieved normal values within the first 60 days after transplantation. DC recovery was dominated by the DC2(-) subset. NK-cell phenotype was altered and cytotoxicity was lower compared with their donors. EFS was 50 +/- 9% (73 +/- 11% for those in CR1 and 26 +/- 11% for those with advanced disease). Faster DC2(-) recovery was associated with better outcome, especially in the MUD setting. In summary, CD3/CD19-depleted HSCT with fludarabine-based RIC without serotherapy resulted in favorable patient survival, and rapid NK, DC and T-cell recovery. Bone Marrow Transplantation (2012) 47, 1419-1427; doi: 10.1038/bmt.2012.43; published online 12 March 2012
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available