Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 12, Pages 6165-6172Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b00845
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Funding
- Ministerio de Ciencia e Innovacion of the Spanish Government
- Fondo Europeo de Desarrollo Regional (FEDER) of the European Union [CTQ2014-56243-P]
- Secretaria d'Universitats i Recerca of the Departament d'Economia i Coneixement of the Catalan Government [2013FI_B1 00209]
- Australian Research Council [FT130100101]
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The internal standard capillary electrophoresis method (IS-CE) has been implemented in a novel sequential injection-capillary electrophoresis instrument for the high-throughput determination of acidity constants (pK(a)) regardless of aqueous solubility, number of pK(a) values, or structure. This instrument comprises a buffer creation system that automatically mixes within a few seconds four reagents for in situ creation of the separation electrolyte with a pH range of 2-13, ionic strength of 10-100 mM and organic solvent content from 0% to 40%. Combined with 1.2 kV/cm and a short effective length (1.5 cm to the UV detector) fast 20 s electrophoretic separations can be obtained. The low standard deviation of the replicates and the low variation compared to reference values show that this system can accurately determine acidity constants of drugs by IS-CE. A single pK(a) can be determined in 2 min and a set of 20 measurements in half an hour, allowing rapid, simple, and flexible determination of pK(a) values of pharmaceutical targets.
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