Journal
BONE
Volume 56, Issue 1, Pages 220-226Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.bone.2013.05.020
Keywords
MicroRNA; miR-133a; Apoptosis; Bcl-xL; Mcl-1; Osteosarcoma
Categories
Funding
- National Natural Science Foundation of China [81202122, 30973019, 81272942]
- Key Biomedicine Research Programs of Science and Technology Commission in Shanghai [10411956000, 10411960400]
- Natural Science Foundation of Science and Technology Commission in Shanghai [064119605]
Ask authors/readers for more resources
Deregulated microRNAs and their roles in cancer development have attracted much attention. Although miR-133a has been shown to be important in osteogenesis, its roles in osteosarcoma carcinogenesis and progression remain unknown. Hence, we focused on the expression and mechanisms of miR-133a in osteosarcoma development in this study. We found that miR-133a was downregulated in osteosarcoma cell lines and primary human osteosarcoma tissues, and its decrease was significantly correlated with tumor progression and prognosis of the patients. Functional studies revealed that restoration of miR-133a could reduce cell proliferation, promote cell apoptosis, and suppress tumorigenicity in osteosarcoma cell lines. Furthermore, bio-informatic prediction and experimental validation were applied to identify target genes of miR-133a, and the results revealed that the anti-tumor effect of miR-133a was probably due to targeting and repressing of Bcl-xL and Mcl-1 expression. Taken together, our data elucidate the roles of miR-133a in osteosarcoma pathogenesis and implicate its potential in cancer therapy. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available