Journal
JOURNAL OF NUCLEIC ACIDS
Volume 2011, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.4061/2011/857149
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Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan [20092014]
- Hirao Taro Foundation of the Konan University Association for Academic Research
- Long-Range Research Initiative Project of Japan Chemical Industry Association
- Grants-in-Aid for Scientific Research [23651130, 21245040] Funding Source: KAKEN
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Both cellular environmental factors and chemical modifications critically affect the properties of nucleic acids. However, the structure and stability of DNA containing abasic sites under cell-mimicking molecular crowding conditions remain unclear. Here, we investigated the molecular crowding effects on the structure and stability of the G-quadruplexes including a single abasic site. Structural analysis by circular dichroism showed that molecular crowding by PEG200 did not affect the topology of the Gquadruplex structure with or without an abasic site. Thermodynamic analysis further demonstrated that the degree of stabilization of the G-quadruplex by molecular crowding decreased with substitution of an abasic site for a single guanine. Notably, we found that the molecular crowding effects on the enthalpy change for G-quadruplex formation had a linear relationship with the abasic site effects depending on its position. These results are useful for predicting the structure and stability of G-quadruplexes with abasic sites in the cell-mimicking conditions.
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