Journal
BONE
Volume 45, Issue 3, Pages 528-533Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.bone.2009.05.020
Keywords
Wear debris; Osteolysis; MLO-Y4 cell; Tumor necrosis factor alpha; Osteocyte apoptosis
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Funding
- NIH/NIAMS [AR054171]
- Grainger Foundation
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Wear debris-induced osteolysis is purportedly the limiting problem affecting the long term results of joint arthroplasty. Pathogenic effects of wear debris in peri-implant cells such as macrophages, osteoblasts and osteoclasts have been well studied. In contrast, the effects of wear debris on osteocytes, which make up over 90% of all bone cells, remain unknown. We hypothesized that metal implant debris can induce the proinflammatory response in osteocytes. This study demonstrated the effects of cobalt-chromium-molybdenum alloy (Co-Cr-Mo) particles on a well-characterized MLO-Y4 osteocyte cell line. Co-Cr-Mo alloy particle treatment significantly (p<0.05) Lip-regulated tumor necrosis factor alpha (TNF alpha) gene expression after 3 and 6 h and TNF alpha protein production after 24 h, but down-regulated interleukin-6 (IL-6) gene expression after 6 h. Co-Cr-Mo alloy particle treatment also induced osteocyte apoptosis after 24 h. This apoptotic effect was partially (40%) dependent on TNF alpha. Therefore, our results suggest that osteocytes play a role in particle-induced inflammation and bone resorption following total joint arthroplasty by inducing pro-inflammatory cytokines and inducing osteocyte apoptosis. (C) 2009 Elsevier Inc. All rights reserved.
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