Journal
BONE
Volume 44, Issue 5, Pages 1015-1017Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.bone.2009.01.376
Keywords
Microstructure; Osteocyte; FITC; Lacuna; Canaliculi
Categories
Funding
- NIH (NIAMS) [AR052866]
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Bone is a composite porous material with two functional levels of porosity: the vascular porosity that surrounds blood vessels and the lacunar-canalicular porosity that surrounds the osteocytes. Both the vascular porosity and lacunar-canalicular porosity are directly involved in interstitial fluid flow, thought to play in important role in bone's maintenance. Because of the small dimensions of the lacunar-canalicular porosity, interstitial fluid space has been difficult to visualize and quantify. We report a new staining protocol that is reliable and easily reproducible, using fluorescein isothiocyanate (FITC) as a probe visualized by confocal microscopy. Reconstructed FITC-stained cross sections enable effective visualization of bone microstructure and microporosities. This new staining process can be used to analyze interstitial fluid space, providing high-resolution quantification of the vascular pores and the lacunar-canalicular network of cortical and cancellous bone. (C) 2009 Elsevier Inc. All rights reserved.
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