Journal
BMC VETERINARY RESEARCH
Volume 9, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1746-6148-9-89
Keywords
Water buffalo; Placenta; Pregnancy-associated glycoprotein; Purification; Radioimmunoassay; Polyclonal antiserum; Pregnancy diagnosis; N-terminal amino acid sequence
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Funding
- University of Perugia (MURST-COFIN)
- SPW-DGARNE [E2011-118]
- FNRS [CC 1.5155.12]
- FSR-ULg [C-02/66]
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Background: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species. Results: Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n = 33) and non-pregnant (n = 26) water buffalo females. Conclusions: Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards.
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