Carbon Nanopipette Electrodes for Dopamine Detection in Drosophila

Small, robust; sensitive electrodes are desired for in vivo neurotransmitter measurement. Carbon nano-pipettes have been previously manufactured and used for single-cell drug delivery and electrophysiological measurements. Here, a Modified fabrication procedure Was developed: to produce batches of solid carbon nanopipette electrodes (CNPEs) with similar to 250 nm diameter tips, and controllable lengths of exposed carbon, ranging from 5 to 175 mu m. The electrochemical properties,of CNPEs were characterized with fast-scan cyclic voltammetry (PS CV) for the first time. CNPEs were used to detect the electroactive neurotransmitters dopamine, serotonin, and octopamine. CNPEs were significantly more sensitive for serotonin detection than traditional carbon-fiber microelectrodes (CFMEs). Similar to CFMEs, CNPEs have a linear response for dopamine concentrations ranging from 0.1 to 10 mu M and a limit of detection of 25 +/- 5 nM. Recordings with CNPEs were stable for over 3 h when the applied triangle waveform was scanned between -0.4 and +1.3 V vs Ag/AgCl/Cl- at 400 V/s. CNPEs were used to detect endogenous dopamine release in Drosophila larvae using optogenetics, which verified the utility of CNPEs for in vivo neuroscience studies. CNPEs are advantageous because they are 1 order of magnitude smaller in diameter than typical CFMEs and have a sharp, tunable geometry that facilitates penetration and implantation for localized measurements in distinct regions of small organisms, Such as the Drosophila brain.