Journal
PLANT SIGNALING & BEHAVIOR
Volume 6, Issue 7, Pages 1012-1015Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/psb.6.7.15550
Keywords
cell wall trafficking; endocytosis; GPI-anchor; PGIP2; PMEI1; secretion pathway; vacuole fluorescent marker
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Funding
- Regione Puglia [14]
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Recently we have studied the secretion pattern of a pectin methylesterase inhibitor protein (PMEI1) and a polygalacturonase inhibitor protein (PGIP2) in tobacco protoplast using the protein fusions, secGFP-PMEI1 and PGIP2GFP. Both chimeras reach the cell wall by passing through the endomembrane system but using distinct mechanisms and through a pathway distinguishable from the default sorting of a secreted GFP. After reaching the apoplast, secGFP-PMEI1 is stably accumulated in the cell wall, while PGIP2-GFP undergoes endocytic trafficking. Here we describe the final localization of PGIP2-GFP in the vacuole, evidenced by co-localization with the marker Aleu-RFP, and show a graphic elaboration of its sorting pattern. A working model taking into consideration the presence of a regulated apoplast-targeted secretion pathway is proposed.
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