Journal
BMC PLANT BIOLOGY
Volume 18, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12870-018-1387-1
Keywords
Genome editing; CRISPR/Cas9; Strigolactones; Plant architecture; Carotenoids; Carotenoid cleavage dioxygenases; CCD7; Crop improvement Rice engineering
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Funding
- King Abdullah University of Science and Technology
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Background: Precision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering). Results: We used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a reduced height, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography-mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient. Conclusions: Taken together, our results show the potential and feasibility of the use of the CRISPR/Cas9 system for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits.
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