Journal
BMC MICROBIOLOGY
Volume 14, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1471-2180-14-150
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Funding
- National Natural Science Foundation of China [31025019]
- Hi-Tech Research and Development Program of China (863 Planning) [2011AA100902]
- Synergetic Innovation Center of Food Safety and Nutrition
- China Agriculture Research System [CARS-37]
- Special Fund for Agro-scientific Research in the Public Interest [201303085]
- Open Projects of Inner Mongolia Natural Science Foundation [20102010]
- Natural Science Foundation of Inner Mongolia [2013MS1205, 2012MS1207]
- Scientific Research Projects of Institution of Higher Education in Inner Mongolia [NJZY12096]
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Background: Economically, Leuconostoc lactis is one of the most important species in the genus Leuconostoc. It plays an important role in the food industry including the production of dextrans and bacteriocins. Currently, traditional molecular typing approaches for characterisation of this species at the isolate level are either unavailable or are not sufficiently reliable for practical use. Multilocus sequence typing (MLST) is a robust and reliable method for characterising bacterial and fungal species at the molecular level. In this study, a novel MLST protocol was developed for 50 L. lactis isolates from Mongolia and China. Results: Sequences from eight targeted genes (groEL, carB, recA, pheS, murC, pyrG, rpoB and uvrC) were obtained. Sequence analysis indicated 20 different sequence types (STs), with 13 of them being represented by a single isolate. Phylogenetic analysis based on the sequences of eight MLST loci indicated that the isolates belonged to two major groups, A (34 isolates) and B (16 isolates). Linkage disequilibrium analyses indicated that recombination occurred at a low frequency in L. lactis, indicating a clonal population structure. Split-decomposition analysis indicated that intraspecies recombination played a role in generating genotypic diversity amongst isolates. Conclusions: Our results indicated that MLST is a valuable tool for typing L. lactis isolates that can be used for further monitoring of evolutionary changes and population genetics.
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