4.6 Article

The role of interactions between bacterial chaperone, aspartate aminotransferase, and viral protein during virus infection in high temperature environment: the interactions between bacterium and virus proteins

Journal

BMC MICROBIOLOGY
Volume 13, Issue -, Pages -

Publisher

BMC
DOI: 10.1186/1471-2180-13-48

Keywords

Protein interaction; Thermophile; Bacteriophage

Categories

Funding

  1. China Ocean Mineral Resources R D Association [DY125-15-E-01]
  2. Project of State Oceanic Administration, China [201205020-03]
  3. Hi-Tech Research and Development Program of China [2012AA092103]

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Background: The life cycle of a bacteriophage has tightly programmed steps to help virus infect its host through the interactions between the bacteriophage and its host proteins. However, bacteriophage-host protein interactions in high temperature environment remain poorly understood. To address this issue, the protein interaction between the thermophilic bacteriophage GVE2 and its host thermophilic Geobacillus sp. E263 from a deep-sea hydrothermal vent was characterized. Results: This investigation showed that the host's aspartate aminotransferase (AST), chaperone GroEL, and viral capsid protein VP371 formed a linearly interacted complex. The results indicated that the VP371-GroEL-AST complex were up-regulated and co-localized in the GVE2 infection of Geobacillus sp. E263. Conclusions: As reported, the VP371 is a capsid protein of GVE2 and the host AST is essential for the GVE2 infection. Therefore, our study revealed that the phage could use the anti-stress system of its host to protect the virus reproduction in a high-temperature environment for the first time.

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