Journal
BMC MICROBIOLOGY
Volume 10, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1471-2180-10-188
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Funding
- National Science Council (Taipei, Taiwan) [NSC 97-2311-B-008-003-MY3]
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Background: Previous studies in Saccharomyces cerevisiae showed that ALA1 (encoding alanyl-tRNA synthetase) and GRS1 (encoding glycyl-tRNA synthetase) respectively use ACG and TTG as their alternative translation initiator codons. To explore if any other non-ATG triplets can act as initiator codons in yeast, ALA1 was used as a reporter for screening. Results: We show herein that except for AAG and AGG, all triplets that differ from ATG by a single nucleotide were able to serve as initiator codons in ALA1. Among these initiator codons, TTG, CTG, ACG, and ATT had similar to 50% initiating activities relative to that of ATG, while GTG, ATA, and ATC had similar to 20% initiating activities relative to that of ATG. Unexpectedly, these non-AUG initiator codons exhibited different preferences toward various sequence contexts. In particular, GTG was one of the most efficient non-ATG initiator codons, while ATA was essentially inactive in the context of GRS1. Conclusion: This finding indicates that a sequence context that is favorable for a given non-ATG initiator codon might not be as favorable for another.
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