Journal
GENOME BIOLOGY
Volume 14, Issue 6, Pages -Publisher
BMC
DOI: 10.1186/gb-2013-14-6-r61
Keywords
Mapping-by-sequencing; SHOREmapping; genetic mapping; whole; genome sequencing; Arabidopsis thaliana
Funding
- Gottfried Wilhelm Leibniz Award of the DFG
- Max Planck Society
Ask authors/readers for more resources
Mapping-by-sequencing combines genetic mapping with whole-genome sequencing in order to accelerate mutant identification. However, application of mapping-by-sequencing requires decisions on various practical settings on the experimental design that are not intuitively answered. Following an experimentally determined recombination landscape of Arabidopsis and next generation sequencing-specific biases, we simulated more than 400,000 mapping-by-sequencing experiments. This allowed us to evaluate a broad range of different types of experiments and to develop general rules for mapping-by-sequencing in Arabidopsis. Most importantly, this informs about the properties of different crossing scenarios, the number of recombinants and sequencing depth needed for successful mapping experiments.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available