Journal
RADIOLOGICAL PHYSICS AND TECHNOLOGY
Volume 6, Issue 1, Pages 7-13Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s12194-012-0163-4
Keywords
Micro-CT; Contrast agent; Mouse spinal cord; Gray matter; White matter
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Histologic methods are destructive and provide only two-dimensional images, whereas three-dimensional information is difficult to obtain. Simple and noninvasive techniques to make up for the shortcomings of histologic experiments are needed. In this study, we investigated the use of micro-CT with a contrast agent for the characterization of fixed mouse spinal cords as a means of assessing micro-structures ex vivo. In addition, we tested the possibility of using contrast agent concentrations for quantitative assessment of ex vivo micro-CT imaging. The spinal cords were soaked in nonionic iodinated contrast agents, and three-dimensional micro-CT was performed. Soaking of the mouse spinal cords in contrast agent resulted in clear differences in signal between the gray matter and the white matter at three planes, and well-defined micro-structures of nerve and bone were observed with the use of threedimensional micro-CT data. We confirmed the potential of ex vivo micro-CT with contrast agent for quantitative assessment of the concentrations.
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