Journal
BMC IMMUNOLOGY
Volume 12, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1471-2172-12-60
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-
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Funding
- National Institute of Health (NIH) [RO1AI59775, RO1AI038985]
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Background: CD4+ T helper type 2 (T(H)2) cells, their cytokines IL-4, IL-5 and IL-13 and the transcription factor STAT6 are known to regulate various features of asthma including lung inflammation, mucus production and airway hyperreactivity and also drive alternative activation of macrophages (AAM). However, the precise roles played by the IL-4/IL-13 receptors and STAT6 in inducing AAM protein expression and modulating specific features of airway inflammation are still unclear. Since T(H)2 differentiation and activation plays a pivotal role in this disease, we explored the possibility of developing an asthma model in mice using T cells that were differentiated in vivo. Results: In this study, we monitored the activation and proliferation status of adoptively transferred allergen-specific naive or in vivo primed CD4+ T cells. We found that both the naive and in vivo primed T cells expressed similar levels of CD44 and IL-4. However, in vivo primed T cells underwent reduced proliferation in a lymphopenic environment when compared to naive T cells. We then used these in vivo generated effector T cells in an asthma model. Although there was reduced inflammation in mice lacking IL-4R alpha or STAT6, significant amounts of eosinophils were still present in the BAL and lung tissue. Moreover, specific AAM proteins YM1 and FIZZ1 were expressed by epithelial cells, while macrophages expressed only YM1 in RAG2(-/-) mice. We further show that FIZZ1 and YM1 protein expression in the lung was completely dependent on signaling through the IL-4R alpha and STAT6. Consistent with the enhanced inflammation and AAM protein expression, there was a significant increase in collagen deposition and smooth muscle thickening in RAG2(-/-) mice compared to mice deficient in IL-4R alpha or STAT6. Conclusions: These results establish that transfer of in vivo primed CD4+ T cells can induce allergic lung inflammation. Furthermore, while IL-4/IL-13 signaling through IL-4R alpha and STAT6 is essential for AAM protein expression, lung inflammation and eosinophilia are only partially dependent on this pathway. Further studies are required to identify other proteins and signaling pathways involved in airway inflammation.
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