Journal
JOURNAL OF EXTRACELLULAR VESICLES
Volume 2, Issue 1, Pages -Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3402/jev.v2i0.19671
Keywords
extracellular vesicles; nanoparticle tracking analysis; light scattering; standardisation
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Funding
- Wellcome Trust Programme Grant [GR079862]
- Wellcome Trust Technology Development Grant [GR087730]
- MRC Programme Grant [MR/J003360/1]
- Oxford Partnership Comprehensive Biomedical Research Centre
- Department of Health's National Institute for Health Research Biomedical Research Centres
- MRC [MR/J003360/1] Funding Source: UKRI
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Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.
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