Journal
BMC IMMUNOLOGY
Volume 11, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1471-2172-11-65
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Funding
- Ministry of Education, Science and Technology [2010-0003291, 2010-0027222]
- Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea
- National Research Foundation of Korea [2010-0003291, 2010-0027222] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Background: Immunization with the spike protein (S) of severe acute respiratory syndrome (SARS)-coronavirus (CoV) in mice is known to produce neutralizing antibodies and to prevent the infection caused by SARS-CoV. Polyethylenimine 25K (PEI) is a cationic polymer which effectively delivers the plasmid DNA. Results: In the present study, the immune responses of BALB/c mice immunized via intranasal (i.n.) route with SARS DNA vaccine (pci-S) in a PEI/pci-S complex form have been examined. The size of the PEI/pci-S nanoparticles appeared to be around 194.7 +/- 99.3 nm, and the expression of the S mRNA and protein was confirmed in vitro. The mice immunized with i.n. PEI/pci-S nanoparticles produced significantly (P < 0.05) higher S-specific IgG1 in the sera and mucosal secretory IgA in the lung wash than those in mice treated with pci-S alone. Compared to those in mice challenged with pci-S alone, the number of B220(+) cells found in PEI/pci-S vaccinated mice was elevated. Co-stimulatory molecules (CD80 and CD86) and class II major histocompatibility complex molecules (I-A(d)) were increased on CD11c(+) dendritic cells in cervical lymph node from the mice after PEI/pci-S vaccination. The percentage of IFN-gamma-, TNF-alpha- and IL-2-producing cells were higher in PEI/pci-S vaccinated mice than in control mice. Conclusion: These results showed that intranasal immunization with PEI/pci-S nanoparticles induce antigen specific humoral and cellular immune responses.
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