Journal
JOURNAL OF APICULTURAL SCIENCE
Volume 57, Issue 2, Pages 147-157Publisher
RESEARCH INST POMOLOGY FLORICULTURE, DIV APICULTURE
DOI: 10.2478/JAS-2013-0025
Keywords
antioxidant activity; beebread; chemical composition; cytotoxicity; glioblastoma multiforme
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Funding
- National Research Committee, Cracow, Poland [N N405 625438]
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Beebread is processed pollen stored in the cells of the honeycomb, with the addition of various enzymes and honey, which undergoes lactic acid fermentation. Ethanolic extracts (EBBs) were obtained from three different samples of beebread from Poland. Assays were carried out for the determination of chemical composition (GC/MS), for the total phenolic content, and for the antioxidant and cytotoxic activities. The effects of beebread extracts (10, 20, 30, 50, 100 mu g/ml) on the viability of the glioblastoma cell line (U87MG) were studied after 24 h, 48 h, and 72 h. Our results indicated a time-dependent inhibitory effect on the viability of U87MG cells treated EBB. The main inhibitory effect of EBB was observed after 72 h; EBB treatment decreased cell viability to 49 - 66%.
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