Journal
BIOINFORMATICS AND BIOLOGY INSIGHTS
Volume 7, Issue -, Pages 55-72Publisher
SAGE PUBLICATIONS LTD
DOI: 10.4137/BBI.S11064
Keywords
host-microbe interactions; organism identification in DNA sequences; contamination in sequence database; next-generation sequencing; purine degradation
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Funding
- University of Parma
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DNA of apparently recent bacterial origin is found in the genomic sequences of Caenorhabditis angaria and Caenorhabditis remanei. Here we present evidence that the DNA belongs to a single species of the genus Leucobacter (high-GC Gram+ Actinobacteria). Metagenomic tools enabled the assembly of the contaminating sequences in a draft genome of 3.2 Mb harboring 2,826 genes. This information provides insight into a microbial organism intimately associated with Caenorhabditis as well as a solid basis for the reassignment of 3,373 metazoan entries of the public database to a novel bacterial species (Leucobacter sp. AEAR). The application of metagenomic techniques can thus prevent annotation errors and reveal unexpected genetic information in data obtained by conventional genomics.
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