Journal
JOURNAL OF EXTRACELLULAR VESICLES
Volume 2, Issue 1, Pages -Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3402/jev.v2i0.19861
Keywords
extracellular vesicles; nano-particle tracking; vesicle counting; sample purity
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Funding
- Cancer Research Wales programme grant
- Movember GAP1 Exosome Biomarker study
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We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.
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