Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 53, Issue 10, Pages 3148-3154Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01449-15
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Funding
- United Kingdom Ministry of Defense (Programme Office)
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Rapid inactivation of Ebola virus (EBOV) is crucial for high-throughput testing of clinical samples in low-resource, outbreak scenarios. The EBOV inactivation efficacy of Buffer AVL (Qiagen) was tested against marmoset serum (EBOV concentration of 1 x 10(8) 50% tissue culture infective dose per milliliter [TCID50.ml(-1)]) and murine blood (EBOV concentration of 1 x 10(7) TCID50.ml(-1)) at 4:1 vol/vol buffer/sample ratios. Posttreatment cell culture and enzyme-linked immunosorbent assay (ELISA) analysis indicated that treatment with Buffer AVL did not inactivate EBOV in 67% of samples, indicating that Buffer AVL, which is designed for RNA extraction and not virus inactivation, cannot be guaranteed to inactivate EBOV in diagnostic samples. Murine blood samples treated with ethanol (4:1 [vol/vol] ethanol/sample) or heat (60 degrees C for 15 min) also showed no viral inactivation in 67% or 100% of samples, respectively. However, combined Buffer AVL and ethanol or Buffer AVL and heat treatments showed total viral inactivation in 100% of samples tested. The Buffer AVL plus ethanol and Buffer AVL plus heat treatments were also shown not to affect the extraction of PCR quality RNA from EBOV-spiked murine blood samples.
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