Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 53, Issue 4, Pages 1406-1410Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.03613-14
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Funding
- U.S. Department of Energy
- CDC
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A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reassessed as other closely related viruses are identified.
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