Journal
BMC GENOMICS
Volume 9, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1471-2164-9-246
Keywords
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Funding
- NCI NIH HHS [CA98699, CA85859, CA111244, R21 CA098699, P01 CA085859, U01 CA111244] Funding Source: Medline
- NIDDK NIH HHS [DK63630, DK069690, U01 DK063630, R01 DK069690] Funding Source: Medline
- NIGMS NIH HHS [P50 GM076547] Funding Source: Medline
- PHS HHS [PM50 GMO76547] Funding Source: Medline
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Background: Expression levels of mRNA and protein by cell types exhibit a range of correlations for different genes. In this study, we compared levels of mRNA abundance for several cluster designation (CD) genes determined by gene arrays using magnetic sorted and laser-capture microdissected human prostate cells with levels of expression of the respective CD proteins determined by immunohistochemical staining in the major cell types of the prostate -basal epithelial, luminal epithelial, stromal fibromuscular, and endothelial -and for prostate precursor/stem cells and prostate carcinoma cells. Immunohistochemical stains of prostate tissues from more than 50 patients were scored for informative CD antigen expression and compared with cell-type specific transcriptomes. Results: Concordance between gene and protein expression findings based on 'present' vs. 'absent' calls ranged from 46 to 68%. Correlation of expression levels was poor to moderate (Pearson correlations ranged from 0 to 0.63). Divergence between the two data types was most frequently seen for genes whose array signals exceeded background (> 50) but lacked immunoreactivity by immunostaining. This could be due to multiple factors, e. g. low levels of protein expression, technological sensitivities, sample processing, probe set definition or anatomical origin of tissue and actual biological differences between transcript and protein abundance. Conclusion: Agreement between these two very different methodologies has great implications for their respective use in both molecular studies and clinical trials employing molecular biomarkers.
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