Journal
JOURNAL OF CLINICAL LABORATORY ANALYSIS
Volume 30, Issue 5, Pages 359-367Publisher
WILEY
DOI: 10.1002/jcla.21863
Keywords
cellulose acetate membrane electrophoresis; kidney; proteome; SDS-PAGE; urinary protein
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Funding
- Bunkyo Gakuin University Research Fund
- Japan Society for the Promotion of Science [23790627]
- Grants-in-Aid for Scientific Research [23790627] Funding Source: KAKEN
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Background: Analysis of urinary proteins using cellulose acetate membrane electrophoresis (CAME) is a useful and challenging method for the recognition of damaged sites in the kidney. However, protein content of each CAME fraction is still not completely understood. Methods: In this study, an effective method of protein extraction from each band fractionated by CAME was established, which enabled us to examine the extracted proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry. Results: Proteins were extracted from the gel and analyzed by mass spectrometry. In all, 31 proteins were identified, including 20 urinary proteins that were newly identified in the CAME-based analysis. Conclusion: This methodology was useful for identifying the proteins responsible for creating unique bands on CAME in a urine sample of a patient with drug-induced interstitial nephritis. These findings provide in-depth characterization of urinary protein contents in each CAME fraction. (C) 2015 Wiley Periodicals, Inc.
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