Journal
CLINICAL PROTEOMICS
Volume 11, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1559-0275-11-16
Keywords
Two dimensional electrophoresis; Advances; Basics; Review
Categories
Funding
- JSPS (Japan Society for Promotion of Science) from Ministry of Education, Culture, Sports, Science and Technology of Japan [23790933]
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Gel-based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional-polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to simultaneously fractionate, identify, and quantify proteins when coupled with mass spectrometric identification or other immunological tests. Although 2-DE was first introduced more than three decades ago, several challenges and limitations to its utility still exist. This review discusses the principles of 2-DE as well as both recent methodological advances and new applications.
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