Journal
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
Volume 14, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1472-6882-14-231
Keywords
Myagropsis myagroides; Sargachromenol; Pro-inflammatory cytokine; Microglia; Nuclear factor-kappa B; Neuroinflammation
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Funding
- National Fisheries Research and Development Institute, Republic of Korea [RP-2013-FS-000]
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Background: Excessive pro-inflammatory cytokine production from activated microglia contributes to neurodegenerative diseases, thus, microglial inactivation may delay the progress of neurodegeneration by attenuating the neuroinflammation. Among 5 selected brown algae, we found the highest antioxidant and anti-neuroinflammatory activities from Myagropsis myagroides ethanolic extract (MME) in lipopolysaccharide (LPS)-stimulated BV-2 cells. Methods: The levels of nitric oxide (NO), prostaglandin E-2 (PGE(2)), and pro-inflammatory cytokines were measured by Griess assay and enzyme linked immunesorbent assay. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinases (MAPKs), and Akt were measured using Western blot. Nuclear translocation and transcriptional activation of nuclear factor-kappa B (NF-kappa B) were determined by immunefluorescence and reporter gene assay, respectively. Results: MME inhibited the expression of iNOS and COX-2 at mRNA and protein levels, resulting in reduction of NO and PGE(2) production. As a result, pro-inflammatory cytokines were reduced by MME. MME also inhibited the activation and translocation of NF-kappa B by preventing inhibitor kappa B-alpha (I kappa B-alpha) degradation. Moreover, MME inhibited the phosphorylation of extracellular signal regulated kinases (ERKs) and c-Jun N-terminal kinases (JNKs). Main anti-inflammatory compound in MME was identified as sargachromenol by NMR spectroscopy. Conclusions: These results indicate that the anti-inflammatory effect of sargachromenol-rich MME on LPS-stimulated microglia is mainly regulated by the inhibition of I kappa B-alpha/NF-kappa B and ERK/JNK pathways.
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