Journal
BMC CANCER
Volume 11, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1471-2407-11-338
Keywords
P3155; HIF-1 alpha?a; prostate cancer; PI3K
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Funding
- Piramal Life Sciences Limited, Goregaon, Mumbai, India
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Background: Hypoxia-inducible factor-1 (HIF-1) is a master regulator of the transcriptional response to hypoxia. It is essential for angiogenesis and is associated with tumor progression and overexpression of HIF-1 alpha has been demonstrated in many common human cancers. Therefore, HIF-1 alpha is one of the most compelling anticancer targets. Methods: To identify HIF-1 alpha inhibitors, luciferase reporter gene assay under hypoxia and normoxia was used. Detailed studies such as western blotting, RT-PCR, immunofluorescence were carried out to elucidate its mechanism of action. Antiangiogenic activity of P3155 was demonstrated by migration assay and tube formation assay. Efficacy study of P3155 was performed on PC-3 xenograft model. Results: P3155 showed specific HIF-1 alpha inhibition with IC50 of 1.4 mu M under hypoxia. It suppressed HIF-1 alpha expression as well as PI3K/Akt pathway and abrogated expression of HIF-1-inducible gene viz. vascular endothelial growth factor (VEGF). P3155 in combination with HIF-1 alpha siRNA showed significant synergistic effect. In addition, it demonstrated significant in vivo efficacy and antiangiogenic potential in prostate cancer cell lines. Conclusion: We have identified a novel HIF-1 alpha inhibitor P3155 that also modulates PI3K/Akt pathway, which may contribute to its significant in vitro and in vivo antitumor activity.
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