Journal
BMC BIOTECHNOLOGY
Volume 14, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1472-6750-14-51
Keywords
Streptomyces; Cloning; Integration vector; Serine integrase; Bacteriophage; SV1
Categories
Funding
- Egyptian Ministry of Higher Education
- University of York
- Biotechnology and Biological Science Research Council, UK [BB/K003356, BB/H001212]
- BBSRC [BB/K003356/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K003356/1] Funding Source: researchfish
Ask authors/readers for more resources
Background: Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. Results: We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. Conclusion: pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
