Journal
BMC BIOTECHNOLOGY
Volume 13, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1472-6750-13-74
Keywords
Protein expression; Glutamine synthetase; Chinese hamster ovary cells; IRES; HEK 293S
Categories
Funding
- Medical Research Council
- Biotechnology and Biological Sciences Research Council
- Wellcome Trust [075491/Z/04]
- MRC [G0600371, MR/K018779/1, MC_UU_12010/4] Funding Source: UKRI
- Medical Research Council [MR/K018779/1, G0600371, MC_UU_12010/4] Funding Source: researchfish
- National Institute for Health Research [DHCS/04/G121/68] Funding Source: researchfish
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Background: The glutamine synthetase-based protein expression system is widely used in industry and academia for producing recombinant proteins but relies on the cloning of transfected cells, necessitating substantial investments in time and handling. We streamlined the production of protein-producing cultures of Chinese hamster ovary cells using this system by co-expressing green fluorescent protein from an internal ribosomal entry site and selecting for high green fluorescent protein-expressing cells using fluorescence-activated cell sorting. Results: Whereas other expression systems utilizing green fluorescent protein and fluorescence-activated cell sorting-based selection have relied on two or more sorting steps, we obtained stable expression of a test protein at levels >50% of that of an average clone and similar to 40% that of the best clone following a single sorting step. Versus clone-based selection, the principal savings are in the number of handling steps (reduced by a third), handling time (reduced by 70%), and the time needed to produce protein-expressing cultures (reduced by similar to 3 weeks). Coupling the glutamine synthetase-based expression system with product-independent selection in this way also facilitated the production of a hard-to-assay protein. Conclusion: Utilizing just a single fluorescence-activated cell sorting-based selection step, the new streamlined implementation of the glutamine synthetase-based protein expression system offers protein yields sufficient for most research purposes, where <10 mg/L of protein expression is often required but relatively large numbers of constructs frequently need to be trialed.
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