Journal
BMC BIOLOGY
Volume 8, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1741-7007-8-142
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Funding
- University of Cologne, Cologne, Germany [1110]
- Deutsche Forschungsgemeinschaft [SFB670]
- International Graduate School in Genetics and Functional Genomics
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Background: Invariant natural killer T (iNKT) cells differ from other T cells by their hyperactive effector T-cell status, in addition to the expression of NK lineage receptors and semi-invariant T-cell receptors. It is generally agreed that the immune phenotype of iNKT cells is maintained by repeated activation in peripheral tissues although no explicit evidence for such iNKT cell activity in vivo has so far been reported. Results: We used an interferon (IFN)-gamma-inducible cytoplasmic protein, Irga6, as a histological marker for local IFN-gamma production. Irga6 was intensely expressed in small foci of liver parenchymal cells and kidney tubular epithelium. Focal Irga6 expression was unaffected by germ-free status or loss of TLR signalling and was totally dependent on IFN-gamma secreted by T cells in the centres of expression foci. These were shown to be iNKT cells by diagnostic T cell receptor usage and their activity was lost in both CD1 d and J alpha-deficient mice. Conclusions: This is the first report that supplies direct evidence for explicit activation events of NKT cells in vivo and raises issues about the triggering mechanism and consequences for immune functions in liver and kidney.
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