A new electrodynamic balance (EDB) design for low-temperature studies: application to immersion freezing of pollen extract bioaerosols

In this paper we describe a newly designed cold electrodynamic balance(CEDB) system, built to study the evaporation kinetics and freezing properties of supercooled water droplets. The temperature of the CEDB chamber at the location of the levitated water droplet can be controlled in the range -40 to +40 A degrees C, which is achieved using a combination of liquid nitrogen cooling and heating by positive temperature coefficient heaters. The measurement of liquid droplet radius is obtained by analysing the Mie elastic light scattering from a 532 nm laser. The Mie scattering signal was also used to characterise and distinguish droplet freezing events; liquid droplets produce a regular fringe pattern, whilst the pattern from frozen particles is irregular. The evaporation rate of singly levitated water droplets was calculated from time-resolved measurements of the radii of evaporating droplets and a clear trend of the evaporation rate on temperature was measured. The statistical freezing probabilities of aqueous pollen extracts (pollen washing water) are obtained in the temperature range -4.5 to -40 A degrees C. It was found that that pollen washing water from water birch (Betula fontinalis occidentalis) pollen can act as ice nuclei in the immersion freezing mode at temperatures as warm as -22.45 (+/- 0.65) A degrees C. Furthermore it was found that the protein-rich component of the washing water was significantly more ice-active than the non-proteinaceous component.