Journal
BMB REPORTS
Volume 46, Issue 8, Pages 410-415Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI: 10.5483/BMBRep.2013.46.8.014
Keywords
Curcumin; HO-1; ICAM-1; Inflammation; Keratinocyte
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Funding
- National Research Foundation of Korea [2009-0093812, 2012 R1A1A4A01007173]
- Ministry of Education, Science and Technology
- Hallym University [HRF-201209-018]
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Adhesion molecules such as ICAM-1 are important in the infiltration of leukocytes into the site of inflammation. In this study, we investigated the inhibitory effects of curcumin on ICAM-1 expression and monocyte adhesiveness as well as its underlying action mechanism in the TNF-alpha-stimulated keratinocytes. Curcumin induced expression of heme oxygenase-1 (HO-1) in the human keratinocyte cell line HaCaT. In addition, curcumin induced Nrf2 activation in dose- and time-dependent manners in the HaCaT cells. Curcumin suppressed TNF-alpha-induced ICAM-1 expression and subsequent monocyte adhesion, which were reversed by the addition of tin protoporphyrin IX (SnPP), a specific inhibitor of HO-1, or HO-1 knockdown using siRNA. Furthermore, Nrf2 knockdown using siRNA reversed the inhibitory effect of curcumin on the TNF-alpha-induced ICAM-1 expression and adhesion of monocytes to keratinocytes. These results suggest that curcumin may exert its anti-inflammatory activity by suppressing the TNF-alpha-induced ICAM-1 expression and subsequent monocyte adhesion via expression of HO-1 in the keratinocytes.
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