Journal
BMB REPORTS
Volume 42, Issue 1, Pages 41-46Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI: 10.5483/BMBRep.2009.42.1.041
Keywords
AU-rich element; Electrophoretic mobility shift assay; Flavonoids; HuC; Natural compounds
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Funding
- Korea Ministry of Education and Human Resources Development
- SNVH Research fund [03-2008-017]
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Post-transcriptional regulation of mRNA stability by Hu proteins is an important mechanism for tumorigenesis. We focused on the molecular interactions between the HuC protein and AU-rich elements (AREs) to find chemical inhibitors of RNA-protein interactions using RNA electrophoretic mobility shift assay with non-radioactive probes. Screening of 52 natural compounds identified 14 candidate compounds that displayed potent inhibitory activity. Six (quercetin, myricetin, (-)-epigallocatechin gallate, ellagic acid, (-)-epicatechin gallate, and rhamnetin) were categorized as phytochemicals, and their IC50 values were low (0.2-1.8 mu M). [BMB reports 2009; 42(1): 41-46]
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