Journal
BMB REPORTS
Volume 42, Issue 8, Pages 516-522Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
DOI: 10.5483/BMBRep.2009.42.8.516
Keywords
Cell proliferation; Estrogen receptor; PKC; PLC-gamma; SH2D4A
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Funding
- National Natural Science Foundation of China [30370785]
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SH2D4A, comprising a single SH2 domain, is a novel protein of the SH2 signaling protein family. We have previously demonstrated SH2D4A is expressed ubiquitously in various tissues and is located in the cytoplasm. In this study we investigated the function of SH2D4A in human embryonic kidney (HEK) 293 cells using interaction analysis, cell proliferation assays, and kinase activity detection. SH2D4A was found to directly bind to estrogen receptor a (ER alpha), and prevent the recruitment of phospholipase C-gamma (PLC-gamma) to ER alpha. Moreover, we observed its inhibitory effects on estrogen-induced cell proliferation, involving the protein kinase C (PKC) signaling pathway. Together, these findings suggested that SH2D4A inhibited cell proliferation by suppression of the ER alpha/PLC-gamma/PKC signaling pathway. SH2D4A may be useful for the development of a new anticancer drug acting as an ER signaling modulator. [BMB reports 2009; 42(8): 516-522]
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