Journal
BLOOD CELLS MOLECULES AND DISEASES
Volume 51, Issue 2, Pages 98-103Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bcmd.2013.03.005
Keywords
Thalassemia; miR-210; Erythropoiesis; Hypoxia; Globin
Categories
Funding
- Office of the Higher Education Commission and Mahidol University under the National Research University Initiative
- Thailand Research Fund (TRF)
- Research Chair Grant
- National Science and Technology Development Agency, Thailand (NSTDA)
- Japan Society for the Promotion of Science (JSPS)
- Strategic Scholarship Fellowship Frontier Research Networks, Office of the Higher Education Commission and Mahidol University
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Erythropoiesis, a process of erythroid production, is controlled by several factors including oxygen level. In this study, the effect of oxygen tension on erythropoiesis was investigated in K562 erythroleukemic cell line and erythroid progenitor cells derived from normal and beta-thalassemia/hemoglobin (Hb) E individuals. The enhanced erythroid differentiation specific markers including increased levels of alpha-, beta- and gamma-globin gene expressions, numbers of HbF positive cells and the presence of glycophorin A surface marker were observed during cell culture under hypoxic atmosphere. The result also showed that miR-210, one of the hypoxia-induced miRNAs, was up-regulated in K562 and beta-thalassemia/HbE progenitor cells cultured under hypoxic condition. Inhibition of miR-210 expression leads to reduction of the globin gene expression and delayed maturation in K562 and erythroid progenitor cells. This indicated that miR-210 contributes to hypoxia-induced erythroid differentiation in both K562 cells and beta-thalassemia/HbE erythroid progenitor cells. (c) 2013 Elsevier Inc. All rights reserved.
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