Natural Deep Eutectic Solvent Extraction Media for Zingiber officinale Roscoe: The Study of Chemical Compositions, Antioxidants and Anti-microbial Activities

In the present investigation, the extraction of ginger (Zingiber officinale Roscoe) rhizome using a new potential green solvents. 6-Gingerol is the major bioactive constituent responsible for the anti-inflammatory, antitumor and antioxidant activities of ginger. The 6-gingerol related compounds was successfully extracted and isolated from Zingiber officinale Roscoe using Natural deep eutectic solvents (NADES) and compared extraction activity with organic solvents. Different combination of NADES was synthesized using evaporating and heating methods. The gingerol related compounds was characterized by using various instrumental methods like UV spectroscopy, FTIR, HPLC and NMR. The FTIR was confirmed presence of gingerols, shogoals and some other essential oils in different NADES extracts. HPLC analysis showed the major presence of gingerols, shogaols and minor existence of essential oils in ginger extracts. Chemical shift values of gingerol and shogaol structure were well fitted with the H-1 and C-13 NMR spectrum. The antioxidant activity of the NADES extracts was higher than the organic solvent extracts. From different combination of NADES extracts, L-Proline and lactic acid (1:1) combination having a higher antioxidant capacity than other three NADES combinations. Antimicrobial activity of four NADESs, ethanol and chloroform of Zingiber officinale Roscoe rhizome were evaluated using agar disc diffusion methods.