Journal
BLOOD
Volume 123, Issue 9, Pages 1336-1340Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2013-09-529800
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Funding
- Swedish Foundation for Strategic Reseearch [KF10-0009, ICA08-0057]
- Marianne and Marcus Wallenberg Foundation [2010.0112]
- Knut and Alice Wallenberg Foundation [2012.0193]
- Royal Swedish Academy of Science
- Avtal om Lakares Forskningstid grants
- Siv-Inger and Per-Erik Andersson Foundation
- Medical Faculty at Lund University
- Swedish Society of Medicine
- Swedish Foundation for Strategic Research (SSF) [ICA08-0057, KF10-0009] Funding Source: Swedish Foundation for Strategic Research (SSF)
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Molecular characterization of malignant plasma cells is increasingly important for diagnostic and therapeutic stratification in multiple myeloma. However, the malignant plasma cells represent a relatively small subset of bone marrow cells, and need to be enriched prior to analysis. Currently, the cell surface marker CD138 (SDC1) is used for this enrichment, but has an important limitation in that its expression decreases rapidly after sampling. Seeking alternatives to CD138, we performed a computational screen for myeloma plasma cell markers and systematically evaluated 7 candidates. Our results conclusively show that the markers CD319 (SLAMF7/CS1) and CD269 (TNFRSF17/BCMA) are considerably more robust than CD138 and enable isolation of myeloma plasma cells under more diverse conditions, including the samples that have been delayed or frozen. Our results form the basis of improved procedures for characterizing cases of multiple myeloma in clinical practice.
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