Journal
BLOOD
Volume 123, Issue 7, Pages 1079-1089Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2013-08-523233
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Funding
- Danish Cancer Society
- Lundbeck Foundation
- Danish Medical Research Council
- Danielsen Foundation
- Brochner Mortensen Foundation
- Dana Lim Foundation
- Lundbeck Foundation [R17-2007-1726] Funding Source: researchfish
- The Danish Cancer Society [R72-A4487] Funding Source: researchfish
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CCAAT/enhancer binding protein-epsilon (C/EBP-epsilon) is considered a master transcription factor regulating terminal neutrophil maturation. It is essential for expression of secondary granule proteins, but it also regulates proliferation, cell cycle, and maturation during granulopoiesis. Cebpe(-/-) mice have incomplete granulocytic differentiation and increased sensitivity toward bacterial infections. The amount of C/EBP-epsilon messenger RNA (mRNA) increases with maturation from myeloblasts with peak level in myelocytes (MC)/metamyelocytes (MM), when the cells stop proliferating followed by a decline in more mature cells. In contrast, C/EBP-epsilon protein is virtually detectable only in the MC/MM population, indicating that expression in more immature cells could be inhibited by microRNAs (miRNAs). We found that miRNA-130a (miR-130a) regulates C/EBP-epsilon protein expression in both murine and human granulocytic precursors. Overexpression of miR-130a in a murine cell line downregulated C/EBP-epsilon protein and lactoferrin (Ltf), cathelicidin antimicrobial protein (Camp), and lipocalin-2 (Lcn2) mRNA expression giving rise to cells with a more immature phenotype, as seen in the Cebpe(-/-) mouse. Introduction of a C/EBP-epsilon mRNA without target site for miR-130a restored both C/EBP-epsilon production, expression of Camp and Lcn2, and resulted in the cells having a more mature phenotype. We conclude that miR-130a is important for the regulation of the timed expression of C/EBP-epsilon during granulopoiesis.
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