Journal
BLOOD
Volume 122, Issue 6, Pages 932-942Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2013-04-495424
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Funding
- Deutsche Forschungsgemeinschaft
- Romeo and Enrica Invernizzi Foundation
- Cariplo Foundation
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Dendritic cells (DC) have the unique capacities to induce primary T-cell responses. In mice, CD8 alpha+DC are specialized to cross-prime CD8(+) T cells and produce interleukin-12 (IL-12) that promotes cytotoxicity. Human BDCA-3(+) DC share several relevant characteristics with CD8 alpha+DC, but the capacities of human DC subsets to induce CD8(+) T-cell responses are incompletely understood. Here we compared CD1c(+) myeloid DC (mDC) 1, BDCA-3(+)mDC2, and plasmacytoid DC(pDC) in peripheral blood and lymphoid tissues for phenotype, cytokine production, and their capacities to prime cytotoxic T cells. mDC1 were surprisingly the only human DC that secreted high amounts of IL-12p70, but they required combinational Toll-like receptor (TLR) stimulation. mDC2 and pDC produced interferon-lambda and interferon-alpha, respectively. Importantly, mDC1 and mDC2 required different combinations of TLR ligands to cross-present protein antigens to CD8(+) T cells. pDC were inefficient and also expressed lower levels of major histocompatibility complex and co-stimulatory molecules. Nevertheless, all DC induced CD8(+) memory T-cell expansions upon licensing by CD4(+) T cells, and primed naive CD8(+) T cells following appropriate TLR stimulation. However, because mDC1 produced IL-12, they induced the highest levels of cytotoxic molecules. In conclusion, CD1c(+) mDC1 are the relevant source of IL-12 for naive T cells and are fully equipped to cross-prime cytotoxic T-cell responses.
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