Journal
BLOOD
Volume 119, Issue 26, Pages 6243-6254Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2011-12-396093
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Funding
- Australian Stem Cell Center, Stem Cells Australia
- Juvenile Diabetes Research Foundation
- National Health and Medical Research Council of Australia (NHMRC)
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Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein-coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR mAb, were found in both posterior mesoderm and anterior mesendoderm populations and were enriched in hemangioblast colony-forming cells (Bl-CFC). The addition of APLN peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hematoendothelial genes in differentiating hESCs, and increased the frequency of Bl-CFCs by up to 10-fold. Furthermore, APLN peptide also synergized with VEGF to promote the growth of hESC-derived endothelial cells. These studies identified APLN as a novel growth factor for hESC-derived hematopoietic and endothelial cells. (Blood. 2012; 119(26): 6243-6254)
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