Journal
BLOOD
Volume 117, Issue 19, Pages 5067-5077Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2010-08-304287
Keywords
-
Categories
Funding
- Swedish Research Council (VR)
- Crafoord Foundation
- Swedish Childhood Cancer Foundation
- Gunnar Nilsson's Cancer Foundation
- Lundgren Foundation
- John Persson's Foundation
- ALF
- Skane County Council's Research and Development Foundation
- Swedish Foundation for Strategic Research
Ask authors/readers for more resources
Nonhematopoietic bone marrow mesenchymal stem cells (BM-MSCs) are of central importance for bone marrow stroma and the hematopoietic environment. However, the exact phenotype and anatomical distribution of specified MSC populations in the marrow are unknown. We characterized the phenotype of primary human BM-MSCs and found that all assayable colony-forming units-fibroblast (CFU-Fs) were highly and exclusively enriched not only in the lin(-)/CD271(+)/CD45(-)/CD146(+) stem-cell fraction, but also in lin(-)/CD271(+)/CD45(-)/CD146(-/low) cells. Both populations, regardless of CD146 expression, shared a similar phenotype and genotype, gave rise to typical cultured stromal cells, and formed bone and hematopoietic stroma in vivo. Interestingly, CD146 was up-regulated in normoxia and down-regulated in hypoxia. This was correlated with in situ localization differences, with CD146 coexpressing reticular cells located in perivascular regions, whereas bone-lining MSCs expressed CD271 alone. In both regions, CD34(+) hematopoietic stem/progenitor cells were located in close proximity to MSCs. These novel findings show that the expression of CD146 differentiates between perivascular versus endosteal localization of non-hematopoietic BM-MSC populations, which may be useful for the study of the hematopoietic environment. (Blood. 2011; 117(19): 5067-5077)
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available