Journal
BLOOD
Volume 118, Issue 13, Pages 3708-3714Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2011-01-332676
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Funding
- National Heart, Lung, and Blood Institute of the National Institutes of Health [P01 HL056949]
- Deutsche Forschungsgemeinschaft, Germany [FU 742/1-1]
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Histones are released from dying cells and contribute to antimicrobial defense during infection. However, extracellular histones are a double-edged sword because they also damage host tissue and may cause death. We studied the interactions of histones with platelets. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Hereby fibrinogen crosslinked histone-bearing platelets and triggered microaggregation. Fibrinogen interactions with alpha IIb beta 3 integrins were not required for this process but were necessary for the formation of large platelet aggregates. Infused histones associated with platelets in vivo and caused a profound thrombocytopenia within minutes after administration. Mice lacking platelets or alpha IIb beta 3 integrins were protected from histone-induced death but not from histone-induced tissue damage. Heparin, at high concentrations, prevented histone interactions with platelets and protected mice from histone-induced thrombocytopenia, tissue damage, and death. Heparin and histones are evolutionary maintained. Histones may combine microbicidal with prothrombotic properties to fight invading microbes and maintain hemostasis after injury. Heparin may provide an innate counter mechanism to neutralize histones and diminish collateral tissue damage. (Blood. 2011; 118(13):3708-3714)
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