Journal
BLOOD
Volume 119, Issue 6, Pages 1607-1616Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2011-08-373886
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Funding
- European Research Council
- Deutsche Forschungsgemeinschaft [Exc 147-1, SFB 834]
- LOEWE
- [SFB/TR23]
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MicroRNAs (miRs) are small RNAs that regulate gene expression at the posttranscriptional level. miR-27 is expressed in endothelial cells, but the specific functions of miR-27b and its family member miR27a are largely unknown. Here we demonstrate that overexpression of miR-27a and miR-27b significantly increased endothelial cell sprouting. Inhibition of both miR27a and miR-27b impaired endothelial cell sprout formation and induced endothelial cell repulsion in vitro. In vivo, inhibition of miR-27a/b decreased the number of perfused vessels in Matrigel plugs and impaired embryonic vessel formation in zebrafish. Mechanistically, miR-27 regulated the expression of the angiogenesis inhibitor semaphorin 6A (SEMA6A) in vitro and in vivo and targeted the 3'-untranslated region of SEMA6A. Silencing of SEMA6A partially reversed the inhibition of endothelial cell sprouting and abrogated the repul-sion of endothelial cells mediated by miR27a/b inhibition, indicating that SEMA6Ais a functionally relevant miR-27downstreamtarget regulating endothelial cell repulsion. In summary, we show that miR-27a/b promotes angiogenesis by targeting the angiogenesis inhibitor SEMA6A, which controls repulsion of neighboring endothelial cells. (Blood. 2012; 119(6): 1607-1616)
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