Journal
FRONTIERS IN MARINE SCIENCE
Volume 3, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmars.2016.00203
Keywords
environmental samples; laboratory procedures; sample manipulation; DNA; biomonitoring
Funding
- European Union (7th Framework Program The Ocean of Tomorrow Theme) [308392]
- Basque Water Agency (URA)
- Fundacion Centros Tecnologicos-IG
- AZTI
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Metabarcoding is an accurate and cost-effective technique that allows for simultaneous taxonomic identification of multiple environmental samples. Application of this technique to marine benthic macroinvertebrate biodiversity assessment for biomonitoring purposes requires standardization of laboratory and data analysis procedures. In this context, protocols for creation and sequencing of amplicon libraries and their related bioinformatics analysis have been recently published. However, a standardized protocol describing all previous steps (i.e., processing and manipulation of environmental samples for macroinvertebrate community characterization) is lacking. Here, we provide detailed procedures for benthic environmental sample collection, processing, enrichment for macroinvertebrates, homogenization, and subsequent DNA extraction for metabarcoding analysis. Since this is the first protocol of this kind, it should be of use to any researcher in this field, having the potential for improvement.
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